Metabolomics: a powerful tool for plant physiology studies
Camila Caldana (CTBE, Brazil)
Metabolomics, which represents the chemical composition in a cell, is a powerful tool in deciphering metabolism and bridging the phenotype–genotype. Despite significant advancement in analytic tools, complete coverage of the metabolome will always be constrained by polarity, stability, dynamic range and biological properties of metabolites. Therefore, the optimal choice for an analytic technology will largely depend on the goal of each study and is usually a compromise of selectivity and speed. As plant growth is closely linked to central metabolism network, gas chromatography coupled to mass spectrometry (GC-MS) technology is a gold standard for a comprehensive coverage of primary metabolism pathways (e.g. organic and amino acids, sugars, sugar alcohols, phosphorylated intermediates and lipophilic compounds). This mini-course will provide a broad overview of key issues in metabolomics analysis- from experimental design to data interpretation and it will encompass theoretical lectures and discussions in crucial topics. Examples of the potential of metabolite profiles to predict plant performance as well as in plant molecular physiology will be presented.
- Limite de 30 vagas .
- O valor das inscrições é de R$ 40,00.